Preparation, characterization and in vitro cytotoxicity of BSA-based nanospheres containing nanosized magnetic particles and/or photosensitizer

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS

卷:321

Article; Proceedings Paper

Rodrigues, Marcilene M. A.¹  Simioni, Andreza R.¹  Primo, Fernando L.²  Siqueira-Moura, Marigilson P.²  Morais, Paulo C.³  Tedesco, Antonio C.¹ 

[1] Univ Sao Paulo, Dept Quim, Lab Fotobiol & Fotomed, Fac Filosofia Ciencias & letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Programa Posgrad Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, Brazil

[3] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF, Brazil

关键词: Magnetic fluid;    Magnetic nanoparticle;    Hyperthermia;    Photodynamic therapy;    Silicon(IV) phthalocyanine;   

DOI  :  10.1016/j.jmmm.2009.02.093

来源: Elsevier

【 摘 要 】

This study reports on the preparation, characterization and in vitro toxicity test of a new nano-drug delivery system (NDDS) based on bovine serum albumin (BSA) nanospheres which incorporates surface-functionalized magnetic nanoparticles (MNP) and/or the silicon(IV) phthalocyanine (NzPc). The new NDDS was engineered for use in photodynamic therapy (PDT) combined with hyperthermia (HPT) to address cancer treatment. The BSA-based nanospheres, hosting NzPc, MNP or both (NzPc and MNP), present spherical shape with hydrodynamic average diameter values ranging from 170 to 450 nm and zeta potential of around -23 mV. No difference on the fluorescence spectrum of the encapsulated NzPc was found regardless of the presence of MNP. Time-dependent fluorescence measurements of the encapsulated NzPc revealed a bi-exponential decay for samples incorporating only NzPc and NzPc plus MNP, in the time window ranging from 1.70 to 5.20 ns. The in vitro assay, using human fibroblasts, revealed no cytotoxic effect in all samples investigated, demonstrating the potential of the tested system as a synergistic NDDS. (C) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

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