Radiolabel validation of cortisol in the hair of rhesus monkeys

PSYCHONEUROENDOCRINOLOGY

卷:97

Article

Kapoor, Amita¹  Schultz-Darken, Nancy¹  Ziegler, Toni E.¹ 

[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, 1220 Capitol Court, Madison, WI 53715 USA

关键词: Cortisol;    Hair cortisol;    Hair hormones;    Radiolabel;    Validation;    HPLC-UV;   

DOI  :  10.1016/j.psyneuen.2018.07.022

来源: Elsevier

【 摘 要 】

Analysis of cortisol in hair has become a widespread tool for assessment of hypothalamic-pituitary-adrenal (HPA) axis activity because of its ease of collection and its ability to provide cumulative data over a period of months. In order to meaningfully interpret hair cortisol, however a direct validation by radio-metabolism is required to understand cortisol incorporation into hair. Tritiated [H-3] -cortisol was IV administered to adult rhesus monkeys to determine 1) if [H-3] is found in the hair after injection of [H-3]-cortisol, 2) the growth rate of hair and 3) the form in which cortisol is found in hair. Samples of hair were collected from newly and previously shaved patches at 14-days and 28-days after [H-3]-cortisol injection. Hair was processed by external wash, grinding, and hormone extractions. Samples were separated by high-performance liquid chromatography (HPLC) and fractions were collected and radioactivity assessed. We found [H-3] incorporated into the hair by the 14-day hair collection and no new radioactivity was found by the 28-day collection. Individual hair growth rate was highly variable between monkeys, indicating that the between-subject hair growth patterns were not consistent. Importantly, for the first time, we showed that systemically administered [H-3]-cortisol was found in the hair as [H-3]-cortisol and [H-3]-cortisone, as well as other glucocorticoid metabolites.

【 授权许可】

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