Diffusion kinetics of the glucose/glucose oxidase system in swift heavy ion track-based biosensors

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS

卷:398

Article

Fink, Dietmar^1,2  Vacik, Jiri¹  Hnatowicz, V.¹  Munoz Hernandez, G.²  Garcia Arrelano, H.³  Alfonta, Lital⁴  Kiv, Arik⁵ 

[1] Nucl Phys Inst, Rez 25068, Czech Republic

[2] Univ Autonoma Metropolitana Iztapalapa, Dept Fis, POB 55-534, Mexico City 09340, DF, Mexico

[3] Univ Autanoma Metropolitana Lerma, Div Ciencias Biol & Salud, Dept Ciencias Ambientales, Av Garzas 10, Municipio De Lerma 52005, Estado De Mexic, Mexico

[4] Ben Gurion Univ Negev, Avram & Stella Goldstein Gore Dept Biotechnol Eng, POB 653, IL-84105 Beer Sheva, Israel

[5] Ben Gurion Univ Negev, Dept Mat Engn, POB 653, IL-84105 Beer Sheva, Israel

关键词: Etched ion tracks;    Track radius;    Polymer;    Enzyme;    Diffusion;    Biosensor;   

DOI  :  10.1016/j.nimb.2017.03.050

来源: Elsevier

【 摘 要 】

For understanding of the diffusion kinetics and their optimization in swift heavy ion track-based biosensors, recently a diffusion simulation was performed. This simulation aimed at yielding the degree of enrichment of the enzymatic reaction products in the highly confined space of the etched ion tracks. A bunch of curves was obtained for the description of such sensors that depend only on the ratio of the diffusion coefficient of the products to that of the analyte within the tracks. As hitherto none of these two diffusion coefficients is accurately known, the present work was undertaken. The results of this paper allow one to quantify the previous simulation and hence yield realistic predictions of glucose-based biosensors. At this occasion, also the influence of the etched track radius on the diffusion coefficients was measured and compared with earlier prediction. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free