Evaluation of cell behavior on modified polypropylene with swift heavy ion irradiation

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS

卷:273

Article

Arbeitman, Claudia R.^1,2  Ibanez, Irene L.²  Garcia Bermudez, Gerardo^1,2  Duran, Hebe^1,2  del Grosso, Mariela F.^1,2  Salguero, Noelia¹ 

[1] TANDAR CNEA, RA-1650 Buenos Aires, DF, Argentina

[2] Consejo Nacl Invest Cient & Tecn, CABA, RA-1033 Buenos Aires, DF, Argentina

关键词: Cell adhesion;    Biocompatibility;    Irradiated polymers;    Swift heavy ions;   

DOI  :  10.1016/j.nimb.2011.07.036

来源: Elsevier

【 摘 要 】

Ion beam irradiation is a well known means to change the physico-chemical properties of polymers, and induced bio and citocompatibility in controlled conditions and in selected areas of surface. However, the enhancement of cell adhesion on a modified substrate does not mean that the surface is adequate for functional cells. The purpose of the present work is to study proliferation, changes in cytoskeleton and cell morphology on substrates as a function of irradiation parameters. We irradiated polypropylene with sulfur (S) ion-beam at energies of 110 MeV with fluences between 1 x 10(6) and 2 x 10(10) ions cm(-2). NIH 3T3 cells were cultured on each sample. Cell morphology was observed using phase contrast microscopy and cytoskeleton proteins with fluorescence microscopy. The analysis show different cellular responses as a functions of irradiation parameter, strongly suggests that different underlying substratum can result in distinct types of cytoskeleton reorganization. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free