Regulation of cell death in human fetal and adult ovaries-Role of Bok and Bcl-XL

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:330

Article

Jaaskelainen, Minna^1,2  Nieminen, Anni³  Pokkyla, Reeta-Maria^1,2  Kauppinen, Marjut^4,5  Liakka, Annikki⁶  Heikinheimo, Markku^4,7  Vaskivuo, Tommi E.^1,2  Klefstrom, Juha³  Tapanainen, Juha S.^1,2 

[1] Univ Oulu, Dept Obstet & Gynaecol, Oulu, Finland

[2] Oulu Univ Hosp, Clin Res Ctr, Oulu, Finland

[3] Biomedicum Helsinki, Genome Scale Biol Res Program, Helsinki, Finland

[4] Univ Helsinki, Childrens Hosp, FIN-00014 Helsinki, Finland

[5] Univ Helsinki, Program Womens Hlth, FIN-00014 Helsinki, Finland

[6] Oulu Univ Hosp, Dept Pathol, Oulu, Finland

[7] Washington Univ, Dept Pediat, St Louis, MO USA

关键词: Apoptosis;    Bok;    Bcl-XL;    Human;    Oocyte;    Granulosa cell;   

DOI  :  10.1016/j.mce.2010.07.020

来源: Elsevier

【 摘 要 】

Of eight million oocytes formed in fetal ovaries, only 400 are ovulated and the rest are degraded via apoptosis. Studies in rodents suggest an important role for Bok and Bcl-X-L. in ovarian apoptosis. but their expression patterns and roles in human ovaries are not well known. Protein expression of Bok and Bcl-X-L as well as the death pathway effectors TNF and caspase-3 were determined in an important collection of samples consisting of human fetal and adult ovaries. A penetrant expression of Bok, Bcl-X-L, TNF and full length and cleaved caspase-3 were characterized in fetal ovaries, with specific patterns in oocytes and pre-granulosa/granulosa cells. Bok and Bcl-X-L were detected also in adult ovaries. Lentiviral shRNA delivery demonstrated that loss of Bok markedly reduces vulnerability to apoptosis and, conversely, loss of Bcl-X-L increases apoptosis in human granulosa tumour cell line. The results suggest important roles for Bok and Bcl-X-L in human ovarian development, follicle maturation and apoptosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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