Seawater transfer down-regulates C-type natriuretic peptide-3 expression in prolactin-producing cells of Japanese eel: Negative correlation with plasma chloride concentration

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:507

Article

Katayama, Yukitoshi¹  Wong, Marty Kwok-Shing²  Kusakabe, Makoto³  Fujio, Megumi¹  Takahashi, Natsuki¹  Yaguchi, Miku¹  Tsukada, Takehiro¹ 

[1] Toho Univ, Dept Biomol Sci, 2-2-1 Miyama, Funabashi, Chiba 2748510, Japan

[2] Univ Tokyo, Atmosphere & Ocean Res Inst, Lab Physiol, 5-1-5 Kashiwanoha, Kashiwa, Chiba 2778564, Japan

[3] Shizuoka Univ, Surku, 836 Otani, Shizuoka, Shizuoka 4228529, Japan

关键词: CNP;    Osmoregulation;    Freshwater adaptation;    Euryhaline fish;   

DOI  :  10.1016/j.mce.2020.110780

来源: Elsevier

【 摘 要 】

In euryhaline fishes, atrial and B-type natriuretic peptides are important hormones in hypo-osmoregulation, whereas osmoregulatory functions of C-type natriuretic peptides (CNPs) remain to be investigated. Although four CNP isoforms (CNP1-4) are mainly expressed in the brain, multiorgan expression of CNP3 was found in euryhaline Japanese eel, Anguilla japonica. Here we identified the CNP3-expressing cells and examined their response to osmotic stress in eel. CNP3 was expressed in several endocrine cells: prolactin-producing cells (pituitary), glucagon-producing cells (pancreas), and cardiomyocytes (heart). Pituitary CNP3 expression was the highest among organs and was decreased following seawater transfer, followed by a decrease in the freshwater-adaptating (hyper-osmoregulatory) hormone prolactin. We also showed the negative correlation between CNP3/prolactin expression in the pituitary and plasma Cl- concentration, but not for plasma Na+ concentration. These results suggest that CNP3 in the pituitary (and pancreas) plays a critical role in freshwater adaptation of euryhaline eel together with prolactin.

【 授权许可】

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