REGULATION OF STEROIDOGENESIS IN CRAYFISH MOLTING GLANDS - INVOLVEMENT OF PROTEIN-SYNTHESIS

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:109

Article

DAUPHINVILLEMANT, C ; BOCKING, D ; SEDLMEIER, D

关键词: STEROIDOGENESIS;    REGULATION;    PROTEIN SYNTHESIS;    ECDYSTEROIDS;    CRUSTACEANS;    MOLTING GLANDS;   

DOI  :  10.1016/0303-7207(95)03489-T

来源: Elsevier

【 摘 要 】

The involvement of continuous protein synthesis in the mechanisms of crustacean steroidogenesis was investigated using crayfish molting glands (Y-organs). During intermolt, Y-organ steroidogenic activity is low. Eyestalk ablation initiates premolt which is characterized by a rapid increase in the production of ecdysteroids. In vitro incorporation of [C-14]leucine into TCA-precipitable proteins was measured in Y-organs. A significant increase of de novo protein synthesis was observed in the premolt stage compared to the intermolt stage. On the other hand, cycloheximide totally suppressed protein synthesis within 2 h and simultaneously led to a strong inhibition of the ecdysteroid synthesis. Sinus gland extracts (containing molt inhibiting hormone) also induced both a limited but reproducible inhibition of Y-organ protein synthesis and a pronounced inhibition of ecdysteroid production within 2 h. The results suggest a functional link between protein synthesis in the Y-organ and sustained ecdysteroid production. The analysis of autoradiographs from one-dimensional gel electrophoreses revealed an overall increase in de novo synthesis of glandular proteins in early premolt but also a more specific effect on distinct proteins (increase of 150, 140, 50-60, 22 and 15-18 kDa proteins) which may be more directly involved in the regulation of ecdysteroidogenesis.

【 授权许可】

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