Beta-adrenergic signals regulate adipogenesis of mouse mesenchymal stem cells via cAMP/PKA pathway

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:323

Article

Li, Haifang^1,2,3  Fong, Chichun^3,4  Chen, Yao³  Cai, Guoping^1,2  Yang, Mengsu^3,4 

[1] Tsinghua Univ, Grad Sch Shenzhen, Div Life Sci, Shenzhen 518055, Peoples R China

[2] Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China

[3] City Univ Hong Kong, Shenzhen Key Lab Biochip Res, Shenzhen 518057, Peoples R China

[4] City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China

关键词: Mesenchymal stem cells (MSCs);    Adipogenesis;    beta-Adrenergic receptors (beta-ARs);    cAMP/PKA pathway;   

DOI  :  10.1016/j.mce.2010.03.021

来源: Elsevier

【 摘 要 】

The adipogenic capacity of mesenchymal stem cells (MSCs) and the involvement of beta-adrenergic signals in lipolysis and thermogenesis have been well established. However, little is known about the development of beta-adrenergic receptor (beta-AR) systems and the role of beta-adrenergic signals in adipogenic differentiation of MSCs. In this study, we demonstrated that both the mRNA and protein levels of beta 2- and beta 3-AR were up-regulated following adipogenesis of mouse bone marrow derived MSCs. We also established that beta-AR agonists negatively while antagonists positively affected MSC adipogenesis. Both the beta 2- and beta 3-AR were involved in MSC adipogenesis, with beta 3-AR being the predominant subtype. The effect of beta-ARs on MSC adipogenesis was at least partly mediated via the cAMP/PKA signaling pathway. These findings suggested that MSC is also a target for beta-adrenergic regulation, and beta-adrenergic signaling (major beta 3-signaling) plays a role in MSC adipogenesis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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