Absence of the long noncoding RNA H19 results in aberrant ovarian STAR and progesterone production

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:490

Article

Chen, Yong^1,2  Wang, Jing^1,3  Fan, Yanhong^1,4  Qin, Chunrong^1,5  Xia, Xi^1,6  Johnson, Joshua⁷  Kallen, Amanda N.¹ 

[1] Yale Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT USA

[2] Fujian Med Univ, Sch Basic Med Sci, Dept Human Anat & Histol & Embryol, Fuzhou 350122, Fujian, Peoples R China

[3] Capital Med Univ, Beijing Friendship Hosp, Dept Oncol, Beijing 100050, Peoples R China

[4] Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100191, Peoples R China

[5] Southern Med Univ, Affiliated Shenzhen City Matern & Child Healthcar, Ctr Reprod Med, Shenzhen 518000, Peoples R China

[6] Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China

[7] Univ Colorado Denver, Div Reprod Sci, Dept Obstet & Gynecol, Aurora, CO 80045 USA

关键词: StAR;    H19;    let-7;    Steroidogenesis;    Progesterone;    Long noncoding RNA;    lncRNA;    cAMP;   

DOI  :  10.1016/j.mce.2019.03.009

来源: Elsevier

【 摘 要 】

The steroidogenic acute regulatory protein (STAR) governs the rate-limiting step in steroidogenesis, and its expression varies depending on the needs of the specific tissue. It is well known that tight control of steroid production is essential for multiple processes involved in reproduction. We recently showed that Star is regulated at the posttranscriptional level in vitro by H19 and let-7. Here we demonstrate that this novel regulatory mechanism is functional in vivo, regulated by cAMP, and that loss of H19 not only disrupts ovarian STAR but also results in altered progesterone production in an H19KO mouse model. This work further strengthens the possibility that noncoding-RNA-mediated regulation of STAR may play an important role in the regulation of steroid hormone production, and contributes further to our understanding of the many ways in which this important gene is regulated.

【 授权许可】

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