Alpha-fetoprotein derived synthetic peptides: Assay of an estrogen-modifying regulatory segment

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:118

Article

关键词: alpha-fetoprotein;    peptides;    estrogen;    rodents;    breast;    uterus;   

DOI  :  10.1016/0303-7207(96)03762-8

来源: Elsevier

【 摘 要 】

This study describes the estrogen bioassay of a synthetic peptide fashioned after an amino acid sequence from human alpha-fetoprotein (HAFP). The synthetic peptide (P149), modeled after a portion of the estrogen binding pocket of rat/human AFP chimeras: was produced via F-MOC solid phase chemistry. Bioassay of P149 in the estrogen-sensitive immature rodent uterus demonstrated an anti-estrogenic (40-50% inhibitory) activity in the 23 h but not the 3-4 h uterine response. In contrast to purified HAFP,incubation of the peptide with estrogen was not a prerequisite for inhibitory activity. The estrogen-dependent increase in uterine thrombin and tissue factor, as determined by an enzymatic esterase assay, was inhibited by 30% in rat uterine cytosols. In an in vitro bioassay of estrogen-induced focus formation in MCF-7 human breast cancer cultures: focus development was inhibited by 70% following peptide exposure. The mechanism of the AFP-derived peptide inhibition of estrogen-dependent growth remains to be determined.

【 授权许可】

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