Gene expression profile of prostate cancer cell lines: Effect of nerve growth factor treatment

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:284

Article

Sigala, S.¹  Bodei, S.¹  Missale, C.¹  Zani, D.²  Simeone, C.²  Cunico, S. Cosciani²  Spano, P. F.¹ 

[1] Univ Brescia, Sch Med, Pharmacol Sect, Dept Biomed Sci & Biotechnol, I-25123 Brescia, Italy

[2] Univ Brescia, Sch Med, Div Urol, I-25123 Brescia, Italy

关键词: nerve growth factor;    DU145 cell line;    PC3 cell line;    microarray analysis;    quantitative RT-PCR;   

DOI  :  10.1016/j.mce.2007.12.015

来源: Elsevier

【 摘 要 】

A dysregulation of the nerve growth factor (NGF)-mediated control of prostate cell growth is associated with the malignant progression of prostate epithelial cells. Exogenous NGF induced in prostate cancer (PCa) cell lines DU145 and PC3 the expression of p75(NGFR), accompanied by a reduction of the cell malignancy. The aim of this study was to analyze the profile of NGF-regulated genes the PCa cell line DU145 by using the cDNA microarray technique. NGF treatment of DU145 cells decreased the expression of 52 known genes, while the expression of 40 known genes was increased. NGF treatment of the DU145 cell line modified the expression profile of clusters of genes involved in invasion and metastasis, in cell proliferation and apoptosis, inflammation, cell metabolism and transcriptional activity. Interestingly, NGF induced the same pattern of gene modifications in both PCa cell lines. Data presented here may help to identify gene/proteins that dispose to PCa progression and to assess future markers that could allow the development of new clinic diagnostic and therapeutical approaches. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free