Human glomerular endothelial cells IGFBPs are regulated by IGF-I and TGF-β1

MOLECULAR AND CELLULAR ENDOCRINOLOGY

卷:154

Article

Giannini, S ; Cresci, B ; Pala, L ; Ciucci, A ; Manuelli, C ; Fujita-Yamaguchi, Y ; Cappugi, P ; Rotella, CM

关键词: IGF-I;    TGF-beta;    IGFBP-3;    IGFBP-4;    endothelial cells;   

DOI  :  10.1016/S0303-7207(99)00063-5

来源: Elsevier

【 摘 要 】

The release of insulin-like growth factor binding proteins (IGFBPs) and their regulation in human glomerular endothelial cells (GENC) was characterised. GENC produce IGFBP-4, IGFBP-2 and IGFBP-3 and express mRNA for IGFBP-2 to IGFBP-5. Due to the fact that IGF-I and TGF-beta(1) modulate glomerular hypertrophy, their action on IGFBP release and GENC growth was studied. IGF-I increased IGFBP-3, IGFBP-2 and decreased IGFBP-4, while TGF-beta(1) decreased IGFBP-3 and apparently increased IGFBP4. All of the IGFBPs, except the TGF-beta(1)-regulated IGFBP-4, were modulated at mRNA level. IGF-I stimulated GENC proliferation, while TGF-beta(2) inhibited their growth. It was demonstrated that an IGFBP-3 antibody reduced GENC proliferation. However, rhIGFBP-3 alone had no effect on GENC, but after 48 h pre-incubation the IGF-I stimulated GENC growth was increased, suggesting that IGFBP-3 could modulate the IGF-I induced GENC proliferation. It was concluded that the stimulatory IGFBP-3 and the inhibitory IGFBP-4 could regulate GENC growth, although the IGFBP-3 seems to have a predominant effect in this control. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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