Defects in myoglobin oxygenation in KATP-deficient mouse hearts under normal and stress conditions characterized by near infrared spectroscopy and imaging

INTERNATIONAL JOURNAL OF CARDIOLOGY

卷:149

Article

Jilkina, Olga^1,2  Glogowski, Miriam¹  Kuzio, Bozena¹  Zhilkin, Peter A.¹  Gussakovsky, Eugene¹  Kupriyanov, Valery V.^1,3 

[1] Natl Res Council Canada, Inst Biodiagnost, Winnipeg, MB R3B 1Y6, Canada

[2] Univ Manitoba, Dept Oral Biol, Winnipeg, MB, Canada

[3] Univ Manitoba, Dept Biochem & Med Genet, Winnipeg, MB, Canada

关键词: Near infrared spectroscopic imaging;    Kir6.2 knockout;    Langendorff-perfused mouse heart;    Heterogeneity of myoglobin oxygen saturation;    2,4-dinitrophenol;    Isoproterenol;   

DOI  :  10.1016/j.ijcard.2010.02.009

来源: Elsevier

【 摘 要 】

Background: Disruption of ATP-sensitive potassium (K-ATP) channel activity results in the development of dilated cardiomyopathy in response to different forms of stress, likely due to the underlying metabolic defects. To further understand the role of Kir6.2-containing channels in the development of cardiac disease, we analysed the left ventricular (LV) wall oxygenation and the physiologic responses induced by acute stress in non-dilated Kir6.2(-/-) hearts. Methods: Control (C57BL6) and Kir6.2(-/-) mouse hearts were perfused in constant flow Langendorff mode with Krebs-Henseleit buffer. Myocardial oxygenation was evaluated using a newly developed technique, near infrared spectroscopic imaging (NIRSI) of the myoglobin (Mb) oxygen saturation parameter (OSP, ratio of oxy- to total Mb). Results: 2,4-dinitrophenol (DNP, 50-mu M) and isoproterenol (0.1-mu M) failed to produce a transient vasodilatory response and caused a significant diastolic pressure increase in Kir6.2(-/-) hearts. DNP strongly suppressed contractile function in both groups and induced severe mean OSP decreases in Kir6.2(-/-) hearts. Isoproterenol-induced decreases in OSP were similar despite the lack of contractile function stimulation in the Kir6.2(-/-) group. The index of OSP spatial heterogeneity (relative dispersion, RD) was lower by 15% in the Kir6.2(-/-) group at the baseline conditions. Recovery after stress caused reduction of RD values by 20% (DNP) and 8% (isoproterenol) in controls; however, these values did not change in the Kir6.2(-/-) group. Conclusions: 1) NIRSI can be used to analyse 2-D dynamics of LV oxygenation in rodent models of cardiomyopathy; 2) Kir6.2-containing K-ATP channels play an important role in maintaining myocardial oxygenation balance under acute stress conditions and in post-stress recovery. Crown Copyright (C) 2010 Published by Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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