Microbial community composition in Brazilian stored diesel fuel of varying sulfur content, using high-throughput sequencing

FUEL

卷:189

Article

de Azambuja, Aline Oliboni¹  Bucker, Francielle¹  de Quadros, Patricia Dorr¹  Zhalnina, Kateryna⁴  Dias, Raquel⁴  Vacaro, Bernardo Brito²  Correa, Camila²  Ferrao, Marco Flores²  de Oliveira Camargo, Flavio Anastacio³  Triplett, Eric⁴  Bento, Fatima Menezes¹ 

[1] Univ Fed Rio Grande do Sul, Dept Microbiol Immunol & Parasitol, Fuels & Biofuels Biodeteriorat Lab LAB BIO, Sarmento Leite 500, Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Inst Chem, Dept Inorgan Chem, Bento Goncalves Ave 9500, Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Soil Sci, Bento Goncalves Ave 7712, Porto Alegre, RS, Brazil

[4] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA

关键词: ULSD;    Sulfur content;    Storage;    High-throughput sequencing analysis;    Microbial community;   

DOI  :  10.1016/j.fuel.2016.10.108

来源: Elsevier

【 摘 要 】

The reduction of sulfur content in diesel is a worldwide trend that seeks to minimize the environmental impact arising from the combustion of this fuel in large cities. However, there is no reliable information on the effect of such a reduction on the formation of mainly aerobic microbial biomass in vehicle and storage tanks. The objective of this study was to assess the microbial community structure using next generation sequencing technique, and to evaluate the microbial biomass in pure diesel containing various sulfur concentrations. Storage conditions were simulated in glass flasks containing mineral medium and pure diesel, with varying sulfur content: UHSD (<= 1800 ppm sulfur); HSD (<= 500 ppm sulfur); LSD (<= 50 ppm sulfur) and ULSD (<= 10 ppm sulfur). Were assessed two microbial contamination levels in the diesel: low contamination (similar to 10(3) CFU L-1) and medium contamination (similar to 10(5) bacterial cells and fungal spores mL(-1)). The flasks were incubated at 30 degrees C and monitored every 10 days over 40 days. Microbial biomass formation was observed in all microcosms, but the highest biomass level at all times was in UHSD with medium contamination (p < 0.05). Results from biomass production and proton nuclear magnetic resonance showed that the ULSD, was not prone to microbial contamination probably because has highly branched carbon chain. Overall, 36 different phyla were identified by sequencing of 16S rDNA and four by 18S rDNA, with Firmicutes (similar to 49%), Bacteroidetes (similar to 25%), Actinobacteria (similar to 17%) and Ascomycota (>99%) being the most abundant. Results suggested that bacterial diversity is increased when low sulfur concentrations are present. Our results reveal a complex microbial diversity from 16S rDNA and confirm the existence of the major fungal Phyla (Ascomycota, Basidiomycota, Chytridiomycota and Glomeromycota) with a large fraction of microorganisms as yet undescribed, reinforcing the importance of understanding the fuel environment in establishing control methods of microbial growth in storage tanks and in bioremediation programs for environments contaminated by fuel. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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