Revisiting of the physico-chemical properties of polyelectrolyte multilayers for a fine tuning of the immobilization of bacteria or nanoparticles

THIN SOLID FILMS

卷:713

Article; Proceedings Paper

Gammoudi, I^1,2  Mathelie-Guinlet, M.^1,3  Benabdallah, Z.¹  Morote, F.¹  Kahli, H.¹  Beven, L.⁴  Kalfat, R.⁵  Othmane, A.⁶  Delville, M. H.³  Grauby-Heywang, C.¹  Dejous, C.²  Cohen-Bouhacina, T.¹ 

[1] Univ Bordeaux, LOMA, CNRS, UMR 5798, F-33400 Talence, France

[2] Univ Bordeaux, Bordeaux INP ENSEIRB MATMECA, IMS, CNRS UMR 5218, F-33400 Talence, France

[3] Univ Bordeaux, ICMCB, Bordeaux INP, CNRS,UMR 5026, 87 Ave Dr Albert Schweitzer, F-33608 Pessac, France

[4] Univ Bordeaux, UMR 1332 Biol Fruit & Pathol, INRA, F-33882 Villenave Dornon, France

[5] Inst Natl Rech & Anal Physicochim, LR15INRAO03, LMTA, T-2020 Sidi Thabet, Tunisia

[6] Univ Monastir, Fac Med, Lab Biophys, T-5019 Monastir, Tunisia

关键词: Polyelectrolytes;    Layer-by-layer;    Love wave sensor;    Atomic force microscopy;    Quartz crystal microbalance with dissipation;    Cyclic voltammetry;   

DOI  :  10.1016/j.tsf.2020.138345

来源: Elsevier

【 摘 要 】

Increasingly used in industrial coatings, polyelectrolytes multilayers (PEMs) are self-assembled systems made of the alternate deposition of oppositely charged polymers on substrates, usually built by the traditional layer-by-layer method. Their properties strongly depend on environmental physico-chemical parameters. Due to the variety of conditions used in the literature on the one hand and the diversity of polyelectrolytes systems on the other hand, it remains difficult to bring out general principles, leading now to a lack of a real understanding of the PEM buildup, from the macro- to the nanoscale. Here, combining acoustic and electrochemical methods with atomic force microscopy, in a systematic approach, we uncover the critical role of the deposition protocol in the growth regime of PEMs made of cationic poly (allylamine hydrochloride) and anionic poly(4-styrene sulfonate, sodium). Traditional dipping leads to thick, heterogeneous and relatively isolating PEMs whereas a spin-coating assisted method leads to thinner, homogeneous and more permeable PEMs. We also highlight that the pH and the ionic strength influence not only the electrostatic interactions and polyelectrolyte conformation in solution but also their organization after their adsorption on the substrate. Finally, our easily and rapidly adaptable protocol paves the way for promising potential bio-applications, since PEMs are applied to the bacterial immobilization on substrates or as a coating for nanostructured biosensor transducer.

【 授权许可】

Free