Titanium-silicon oxide film structures for polarization-modulated infrared reflection absorption spectroscopy

THIN SOLID FILMS

卷:517

Article

Dunlop, Iain E.^2,6  Zorn, Stefan¹  Richter, Gunther³  Srot, Vesna⁴  Kelsch, Marion⁴  van Aken, Peter A.⁴  Skoda, Maximilian^1,5  Gerlach, Alexander¹  Spatz, Joachim P.^2,6  Schreiber, Frank¹ 

[1] Univ Tubingen, Inst Appl Phys, D-72076 Tubingen, Germany

[2] Max Planck Inst Met Res, Dept New Mat & Biosyst, D-70569 Stuttgart, Germany

[3] Max Planck Inst Met Res, Cent Sci Facil, Thin Film Lab, D-70569 Stuttgart, Germany

[4] Max Planck Inst Met Res, Stuttgart Ctr Electron Microscopy, D-70569 Stuttgart, Germany

[5] Univ Oxford, Phys & Theoret Chem Lab, Oxford OX1 3QZ, England

[6] Heidelberg Univ, Dept Biophys Chem, D-69120 Heidelberg, Germany

关键词: Biotin-streptavidin binding;    Electron energy loss spectroscopy (EELS);    Fourier transform infrared spectroscopy;    Poly(ethylene glycol);    Protein resistant surfaces;    Silicon oxide;    Titanium;    Transmission electron microscopy (TEM);    X-ray photoelectron spectroscopy (XPS);    X-ray reflectometry;   

DOI  :  10.1016/j.tsf.2008.10.058

来源: Elsevier

【 摘 要 】

We present a titanium-silicon oxide film structure that permits polarization modulated infrared reflection absorption spectroscopy on silicon oxide surfaces. The structure consists of a similar to 6 nm sputtered silicon oxide film on a similar to 200 nm sputtered titanium film. Characterization using conventional and scanning transmission electron microscopy, electron energy loss spectroscopy, X-ray photoelectron spectroscopy and X-ray reflectometry is presented. We demonstrate the use of this structure to investigate a selectively protein-resistant self-assembled monolayer (SAM) consisting of silane-anchored, biotin-terminated poly(ethylene glycol) (PEG). PEG-associated IR bands were observed. Measurements of protein-characteristic band intensities showed that this SAM adsorbed streptavidin whereas it repelled bovine serum albumin, as had been expected from its structure. (C) 2008 Elsevier B.V. All rights reserved.

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