Investigation of the antimicrobial properties of modified multilayer diamond-like carbon coatings on 316 stainless steel

SURFACE & COATINGS TECHNOLOGY

卷:314

Article; Proceedings Paper

Robertson, Shaun N.^1,2  Gibson, Des²  MacKay, William G.¹  Reid, Stuart²  Williams, Craig¹  Birney, Ross² 

[1] Univ West Scotland, Sch Hlth Nursing & Midwifery, Inst Healthcare Policy & Practice, Paisley PA1 2BE, Renfrew, Scotland

[2] Univ West Scotland, SUPA, Sch Engn & Comp, Inst Thin Films Sensors & Imaging, Paisley PA1 2BE, Renfrew, Scotland

关键词: Modified diamond like carbon;    Microbial biofilm prevention;    Anti-biofouling;   

DOI  :  10.1016/j.surfcoat.2016.11.035

来源: Elsevier

【 摘 要 】

Modified diamond-like carbon (DLC) coatings were deposited onto 25 mm diameter 316 stainless steel discs by pulsed (direct current) hollow cathode plasma enhanced chemical vapour deposition (HC-PECVD). Multilayer films of total thickness 1-2 mu m were deposited, both with and without germanium dopant. Characterisation of the coatings was performed by SEM/EDX, surface energy/contact angle analysis, and assessment of possible biofilm-inhibiting properties. Both modified DLC and germanium-doped DLC (Ge-DLC) coatings showed a significant anti-biofouling effect on P. aeruginosa, a Gram-negative bacterium. A 90% reduction in P. aeruginosa biomass was observed compared to control for both DLC and Ge-DLC, however this effect could not be attributed to germanium incorporation alone. Neither modified DLC nor Ge-DLC showed a significant inhibitory effect on S. aureus, a Gram-positive bacterium. Scanning electron microscopy of P. aeruginosa biofilms on Ge-DLC coated 316 stainless steel clearly displayed disruption of the cellular wall, as well as leakage of cellular components; this effect was not observed with modified DLC coating. This suggests that germanium-doped DLC coatings may potentially exhibit a cidal mode of action versus P. aeruginosa biofilms. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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