Development of novel dual-action coatings with osteoinductive and antibacterial properties for 3D-printed titanium implants

SURFACE & COATINGS TECHNOLOGY

卷:403

Article

Rodriguez-Contreras, Alejandra¹  Torres, Diego^1,3  Guillem-Marti, Jordi¹  Sereno, Patricia¹  Pau Ginebra, Maria^1,2  Calero, Jose A.³  Maria Manero, Jose¹  Ruperez, Elisa¹ 

[1] Univ Politecn Catalunya UPC, Dept Mat Sci & Engn, Escola Engn Barcelona Est EEBE, Biomat Biomech & Tissue Engn Grp BBT, Eduard Maristany 16, Barcelona 08019, Spain

[2] Inst Bioengn Catalonia IBEC, Baldiri Reixac 10-12, Barcelona 08028, Spain

[3] AMES Grp, Carrer Laurea Miro 388, Barcelona 08980, Spain

关键词: Porous structures;    Gallium;    Biomaterials;    Antibacterial activity;    Titanium implants;    3D-printing;   

DOI  :  10.1016/j.surfcoat.2020.126381

来源: Elsevier

【 摘 要 】

Gallium (Ga) has been recently proposed as a novel therapeutic agent, since it promotes bone formation and exhibits antibacterial properties. This work focuses on the optimization of a thermochemical treatment that incorporates Ga ions by the addition of the body-friendly Ga nitrate approved by the Food and Drug Administration. The objective was to simultaneously provide the inner and the outer surfaces of porous-titanium surfaces obtained by 3D-printing with bioactivity and antibacterial properties. The apatite-forming ability of the coating, as well as the antibacterial activity and SaOS-2 cell adhesion, proliferation, differentiation and mineralization were evaluated and compared with untreated Ti surfaces. The characterization of the surfaces revealed the presence of a Ga-containing calcium titanate layer, which was non cytotoxic and in simulated body fluid produced a homogeneous apatite coating well adhered to the substrate. The formation of this apatite layer was accelerated with increasing Ga amounts present on the surface, resulting also in an increase in thickness. An initial quick release of Ga ion promoted the antibacterial effect against gram positive strains, especially for Pseudomonas aeruginosa, one of the most frequent resistant pathogens in nosocomial infections. SaOS-2 cells adhered and proliferated on the Ga-doped Ti surfaces, its presence contributed to cell differentiation and to considerably increase the mineralization levels. Thus, the developed multifunctional coatings could provide bioactivity to the porous Ti implants while protecting them from the most frequent gram-negative pathogens.

【 授权许可】

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