Transcriptome Characterization of Estrogen-Treated Human Myocardium Identifies Myosin Regulatory Light Chain Interacting Protein as a Sex-Specific Element Influencing Contractile Function

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY

卷:59

Article

Kararigas, Georgios^1,2  Bito, Virginie⁵  Tinel, Hanna³  Becher, Eva^1,2  Baczko, Istvan⁶  Knosalla, Christoph⁴  Albrecht-Kuepper, Barbara³  Sipido, Karin R.⁵  Regitz-Zagrosek, Vera^1,2 

[1] Charite, Inst Gender Med, D-10115 Berlin, Germany

[2] Charite, Ctr Cardiovasc Res, D-10115 Berlin, Germany

[3] Bayer HealthCare, Wuppertal, Germany

[4] German Heart Inst Berlin, Berlin, Germany

[5] Univ Leuven, Lab Expt Cardiol, Louvain, Belgium

[6] Univ Szeged, Dept Pharmacol & Pharmacotherapy, Szeged, Hungary

关键词: contractility;    estrogen;    gene expression;    gene regulation;    genomics;    sex-specific;   

DOI  :  10.1016/j.jacc.2011.09.054

来源: Elsevier

【 摘 要 】

Objectives This study investigated the effects of 17 beta-estradiol (E2) on gene regulation in human cardiac tissues. We hypothesized that a candidate E2 effect is cardiomyocyte (CM)- and sex-specific, conserved between humans and mice, and that E2 impairs contractile function in male CMs only. Background Both men and women produce E2 locally from androgenic precursors. E2 regulates cardiovascular function, but specific mechanisms, protective or harmful, are not fully understood. Methods We performed genome-wide expression profiling of E2-treated cardiac tissues from men and women, and studied gene expression and function in CMs from hearts of male and female E2-treated mice. Results We found 36 E2-dependent genes regulated in a sex-specific manner. Of these, after E2 exposure, the myosin regulatory light chain interacting protein (MYLIP) gene was induced in tissues of men only. Focusing on Mylip and employing isolated mouse CMs, we confirmed our hypotheses that the E2 effect is CM- and sex-specific and conserved between humans and mice. The E2-treatment led to impaired contractile function in male CMs only, which was characterized by increased Mylip mRNA and protein levels, and decreased myosin regulatory light chain (Mrlc) protein. Our report is the first to our knowledge to show that cardiac Mrlc is an in vivo substrate for Mylip, leading to augmented Mrlc ubiquitination. Of relevance, we found that MYLIP expression levels rise with increasing age in hearts of men. Conclusions E2 directly influences cardiac gene regulation, and E2 actions may be different between the sexes. Since E2 levels rise in older and/or obese men, pharmacological targeting of MYLIP in men with elevated E2 levels could possibly decrease their risk for the development or progression of cardiovascular disease. (J Am Coll Cardiol 2012; 59: 410-7) (C) 2012 by the American College of Cardiology Foundation

【 授权许可】

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