Human Cardiac Progenitor Cells Engineered With Pim-I Kinase Enhance Myocardial Repair

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY

卷:60

Article

Mohsin, Sadia¹  Khan, Mohsin¹  Toko, Haruhiro¹  Bailey, Brandi¹  Cottage, Christopher T.¹  Wallach, Kathleen¹  Nag, Divya²  Lee, Andrew²  Siddiqi, Sailay¹  Lan, Feng²  Fischer, Kimberlee M.¹  Gude, Natalie¹  Quijada, Pearl¹  Avitabile, Daniele¹  Truffa, Silvia¹  Collins, Brett¹  Dembitsky, Walter³  Wu, Joseph C.²  Sussman, Mark A.¹ 

[1] San Diego State Univ, San Diego Heart Res Inst, San Diego, CA 92182 USA

[2] Stanford Univ, Sch Med, Stanford, CA 94305 USA

[3] Sharp Mem Hosp & Rehabil Ctr, San Diego, CA USA

关键词: heart repair;    human cardiac progenitor cells;    Pim-1 kinase;   

DOI  :  10.1016/j.jacc.2012.04.047

来源: Elsevier

【 摘 要 】

Objectives The goal of this study was to demonstrate the enhancement of human cardiac progenitor cell (hCPC) reparative and regenerative potential by genetic modification for the treatment of myocardial infarction. Background Regenerative potential of stem cells to repair acute infarction is limited. Improved hCPC survival, proliferation, and differentiation into functional myocardium will increase efficacy and advance translational implementation of cardiac regeneration. Methods hCPCs isolated from the myocardium of heart failure patients undergoing left ventricular assist device implantation were engineered to express green fluorescent protein (hCPCe) or Pim-1-GFP (hCPCeP). Functional tests of hCPC regenerative potential were performed with immunocompromised mice by using intramyocardial adoptive transfer injection after infarction. Myocardial structure and function were monitored by echocardiographic and hemodynamic assessment for 20 weeks after delivery. hCPCe and hCPCeP expressing luciferase were observed by using bioluminescence imaging to noninvasively track persistence. Results hCPCeP exhibited augmentation of reparative potential relative to hCPCe control cells, as shown by significantly increased proliferation coupled with amelioration of infarction injury and increased hemodynamic performance at 20 weeks post-transplantation. Concurrent with enhanced cardiac structure and function, hCPCeP demonstrated increased cellular engraftment and differentiation with improved vasculature and reduced infarct size. Enhanced persistence of hCPCeP versus hCPCe was revealed by bioluminescence imaging at up to 8 weeks post-delivery. Conclusions Genetic engineering of hCPCs with Pim-1 enhanced repair of damaged myocardium. Ex vivo gene delivery to modify stem cells has emerged as a viable option addressing current limitations in the field. This study demonstrates that efficacy of hCPCs from the failing myocardium can be safely and significantly enhanced through expression of Pim-1 kinase, setting the stage for use of engineered cells in pre-clinical settings. (J Am Coll Cardiol 2012;60:1278-87) (C) 2012 by the American College of Cardiology Foundation

【 授权许可】

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