Toward encoding reactivity using double-stranded DNA. Sequence-dependent native chemical ligation of DNA binding polyamides

TETRAHEDRON

卷:69

Article

Mascarenas, Jose L.¹ 

[1] Univ Santiago de Compostela, Dept Quim Organ, Santiago De Compostela 15782, Spain

关键词: DNA binding;    Native chemical ligation;    Molecular recognition;    Polyamides;   

DOI  :  10.1016/j.tet.2013.05.126

来源: Elsevier

【 摘 要 】

The coupling reaction between distamycin-related polyamides equipped with cysteine and thioester groups can be accelerated in the presence of double-stranded (ds) oligonucleotides with selected sequences. While the coupling reaction of reactive partners containing three pyrrole units is accelerated by dsDNAs containing ATTTTA or ATGTTA sites, the heterodimeric coupling between a tripyrrole and a polyamide equipped with two pyrroles and one imidazole, is accelerated by the latter DNA, but not by the other containing the A/T rich tract. These differences can be exploited for selecting preferred coupling pairs from a mixture of reactive monomers; therefore the reaction outcome depends on the instructions provided by the dsDNA sequence. (C) 2013 Elsevier Ltd. All rights reserved.

【 授权许可】

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