Science based calibration for the extraction of 'analyte-specific' HPLC-DAD chromatograms in environmental analysis

TALANTA

卷:83

Article

Kuligowski, J.¹  Martinez Galera, M.²  Gil Garcia, M. D.²  Culzoni, M. J.³  Goicoechea, H. C.³  Garrigues, S.¹  Quintas, G.¹  de la Guardia, M.¹ 

[1] Univ Valencia, Dept Analyt Chem, E-46100 Burjassot, Spain

[2] Univ Almeria, Fac Ciencias Expt, Dept Hidrogeol & Quim Analit, E-04120 La Canada De San Urbano, Almeria, Spain

[3] Univ Nacl Litoral, Fac Bioquim & Ciencias Biol, LADAQ, Catedra Quim Analit 1, Santa Fe, Argentina

关键词: Science based calibration;    High performance liquid chromatography-diode array detection (HPLC-DAD);    Environmental analysis;   

DOI  :  10.1016/j.talanta.2010.07.060

来源: Elsevier

【 摘 要 】

Multivariate science based calibration (SBC) has been applied to the resolution of overlapped peaks in liquid chromatography with diode array detection (LC-DAD). Complex river water samples spiked with 11 pharmaceutical substances resulted in poorly resolved chromatograms containing additional peaks from interfering matrix compounds and a change in the background absorbance due to the mobile phase gradient. Applying the present multivariate approach it was possible to resolve all 11 analytes from overlapping peaks, obtaining linear calibration lines (R-2 > 0.96). Recovery percentages on spiked samples ranged between 74.6 and 113.5%, which are quite satisfactory taking into account the low concentration ranges considered to 1-7 mu g L-1. (c) 2010 Elsevier B.V. All rights reserved.

【 授权许可】

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