Stable isotope labeling assisted liquid chromatography-tandem mass spectrometry for the analysis of perfluorinated carboxylic acids in serum samples

TALANTA

卷:166

Article

Zhang, Shijuan^1,2  Ji, Zhongyin³  Sun, Zhiwei^1,2  Li, Mingshuang¹  Sheng, Cuncun¹  Yue, Mingbo¹  Yu, Yanxin¹  Chen, Guang^1,2  You, Jinmao^1,2,3 

[1] Qufu Normal Univ, Shandong Province Key Lab Life Organ Analy, Jinan, Peoples R China

[2] Qufu Normal Univ, Kye Lab Pharm Intermed & Anal, Jinan, Peoples R China

[3] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China

关键词: Stable isotope labeling;    PFCA;    Serum;    Fluorescence;   

DOI  :  10.1016/j.talanta.2017.01.069

来源: Elsevier

【 摘 要 】

A new stable isotope labeling (SIL) reagent pair, 10-methyl-acridone-2-sulfonohydrazide (MASH) and its deuterated counterpart d(3)-MASH was synthesized and successfully applied to the analysis of perfluorinated carboxylic acids (PFCAs) in serum samples. The limits of detection (LODs) were in the range of 0.07-0.42 mu g/L, and the limits of quantitation (LOQs) were in the range of 0.25-1.38 mu g/L. Besides ionization enhancing effect, MASH also showed excellent fluorescence property. Therefore, the mass spectrometer operation cost was greatly lowered by carrying out parameter optimization experiments on HPLC which is easier to operate and maintain. The SIL strategy was confirmed to be effective in reducing matrix effect. The developed multiple reaction monitoring (MRM) condition of PFCAs was also suitable for other carboxylic acid due to the introduction of MASH which is more prone to fragmentation than the analytes. With the MRM conditions obtained from PFCAs, fatty acids were also found in serum samples. This feature made the proposed method show powerful potential in the identification of acidic compounds in complex samples in the absence of corresponding standard.

【 授权许可】

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