Determination of thiodicarb using a biosensor based on alfalfa sprout peroxidase immobilized in self-assembled monolayers

TALANTA

卷:82

Article

Moccelini, Sally K.¹  Vieira, Iolanda C.¹  de Lima, Fabio²  Lucca, Bruno G.²  Barbosa, Antonio M. J.²  Ferreira, Valdir S.² 

[1] Univ Fed Santa Catarina, Dept Quim, Lab Biossensores, BR-88040970 Florianopolis, SC, Brazil

[2] Univ Fed Mato Grosso do Sul, Dept Quim, BR-79070900 Campo Grande, MS, Brazil

关键词: Biosensor;    Thiodicarb;    Alfalfa sprouts;    Self-assembled monolayers;   

DOI  :  10.1016/j.talanta.2010.04.015

来源: Elsevier

【 摘 要 】

A biosensor based on alfalfa sprout (Medicago sativa) homogenate as a source of peroxidase is proposed for the determination of thiodicarb by square-wave voltammetry. This enzyme was immobilized in self-assembled monolayers of L-cysteine on a gold electrode. Several parameters were investigated to evaluate the optimum conditions for operation of the biosensor. The analytical curve was linear for thiodicarb concentrations of 2.27 x 10(-6) to 4.40 x 10(-5) mol L-1 with a detection limit of 5.75 x 10(-7) mol L-1. The lifetime of the Au-alfalfa sprout-SAMs was 20 days (at least 220 determinations). The average recovery of thiodicarb from samples of vegetable extracts ranged from 99.02 to 101.04%. The results obtained for thiodicarb in vegetable extracts using the proposed method are in close agreement with those using a high performance liquid chromatography procedure at the 95% confidence level. (C) 2010 Elsevier B.V. All rights reserved.

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