Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control

TALANTA

卷:125

Article

Guo, Feng¹  Shao, Jing²  Liu, Qian¹  Shi, Jian-bo¹  Jiang, Gui-bin¹ 

[1] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China

[2] Natl Inst Sports Med, Beijing 100029, Peoples R China

关键词: Online turboflow solid-phase extraction;    Anabolic androgenic steroids;    Urine;    Clinical monitoring;    Doping control;   

DOI  :  10.1016/j.talanta.2014.03.010

来源: Elsevier

【 摘 要 】

A novel method for automated and sensitive analysis of testosterone, androstenedione, methyltestosterone and methenolone in urine samples by online turbulent flow solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry was developed. The optimization and validation of the method were discussed in detail. The Turboflow C18-P SPE column showed the best extraction efficiency for all the analytes. Nanogram per liter (ng/L) level of MS could be determined directly and the limits of quantification (LOQs) were 0.01 ng/mL, which were much lower than normally concerned concentrations for these typical anabolic androgenic steroids (AAS) (0.1 ng/mL). The linearity range was from the LOQ to 100 ng/mL for each compound, with the coefficients of determination (r(2)) ranging from 0.9990 to 0.9999. The intraday and interday relative standard deviations (RSDs) ranged from 1.1% to 14.5% (n=5). The proposed method was successfully applied to the analysis of urine samples collected from 24 male athletes and 15 patients of prostate cancer. The proposed method provides an alternative practical way to rapidly determine MS in urine samples, especially for clinical monitoring and doping control. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

Free