Development of an automatic solid phase extraction and liquid chromatography mass spectrometry method by using a monolithic column for the analysis of Cyclosporin A in human plasma

TALANTA

卷:79

Article

Alvarez, Covadonga²  Wainer, Irving W.¹ 

[1] NIH, Lab Bioanalyt Chem, Gerontol Res Ctr, Baltimore, MD 21224 USA

[2] Univ Complutense Madrid, Fac Pharm, Dept Pharmaceut Technol, E-28040 Madrid, Spain

关键词: Cyclosporin A;    Monolithic columns;    LC-ESI-MS;   

DOI  :  10.1016/j.talanta.2009.03.054

来源: Elsevier

【 摘 要 】

A sensitive and specific and automated liquid chromatography-electrospray mass spectrometric (LC-ESI-MS) assay for the quantification of Cyclosporin A in human plasma was developed. Following a simple protein precipitation step, the supernatant was extracted on-line and directly injected into the system LC-ESI-MS. A relatively new type of monolithic column consisting of a silica rod with bimodal pore structure was used to achieve a retention time of 2.4 min with a very low backpressure at a flow rate of 1 ml/min. The assay was linear from 0.050 to 1.000 mu g/ml. The mean recovery was 91%. The mean inter-day and intra-day precisions were 1.85% and 2.83%, respectively. The combination of the automated solid phase extraction and the low retention time achieved with this columns increase the throughput and decrease the time of analysis of each sample. This technology is useful in order to improve the efficiency of the bioanalytical studies. Published by Elsevier B.V.

【 授权许可】

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