Sample preparation for an optimized extraction of localized metabolites in lichens: Application to Pseudevernia furfuracea

TALANTA

卷:150

Article

Komaty, Sarah^1,3  Letertre, Marine^1,3  Dang, Huyen Duong^1,3  Jungnickel, Harald⁴  Laux, Peter⁴  Luch, Andreas⁴  Carrie, Daniel^1,3  Merdrignac-Conanec, Odile^1,3  Bazureau, Jean-Pierre^1,3  Gauffre, Fabienne^1,3  Tomasi, Sophie^2,3  Paquin, Ludovic^1,3 

[1] Univ Rennes 1, Inst Sci Chim Rennes, UMR 6226, ICMV, Campus Beaulieu,263 Ave Gen Leclerc, F-35042 Rennes, France

[2] Univ Rennes 1, Inst Sci Chim Rennes, UMR 6226, PNSCM, 2 Ave Pr Leon Bernard, F-35043 Rennes, France

[3] Univ Europeenne Bretagne, Bretagne, France

[4] German Fed Inst Risk Assessment, Max Dohrn Str 8-10, D-10589 Berlin, Germany

关键词: Microwave extraction;    Lichen;    Secondary metabolite;    Grinding process;    TOF-SIMS;   

DOI  :  10.1016/j.talanta.2015.12.081

来源: Elsevier

【 摘 要 】

Lichens are symbiotic organisms known for producing unique secondary metabolites with attractive cosmetic and pharmacological properties. In this paper, we investigated three standard methods of preparation of Pseudevernia furfuracea (blender grinding, ball milling, pestle and mortar). The materials obtained were characterized by electronic microscopy, nitrogen adsorption and compared from the point of view of extraction. Their microscopic structure is related to extraction efficiency. In addition, it is shown using thalline reactions and mass spectrometry mapping (TOF-SIMS) that these metabolites are not evenly distributed throughout the organism. Particularly, atranorin (a secondary metabolite of interest) is mainly present in the cortex of P. furfuracea. Finally, using microwave assisted extraction (MAE) we obtained evidence that an appropriate preparation can increase the extraction efficiency of atranorin by a factor of five. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

Free