A label-free fluorescent aptasensor for the detection of Aflatoxin B1 in food samples using AIEgens and graphene oxide

TALANTA

卷:198

Article

Jia, Yongmei¹  Wu, Feng²  Liu, Peilian¹  Zhou, Guohua¹  Yu, Biao¹  Lou, Xiaoding²  Xia, Fan^2,3 

[1] Lingnan Normal Univ, Sch Chem & Chem Engn, Zhanjiang 524048, Peoples R China

[2] China Univ Geosci, Fac Mat Sci & Chem, Wuhan 430074, Peoples R China

[3] Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China

关键词: Aflatoxin B1;    AIEgens;    Graphene Oxide;    Aptamer;    Label-free;   

DOI  :  10.1016/j.talanta.2019.01.078

来源: Elsevier

【 摘 要 】

The detection of Aflatoxin B1 (AFB1) has attracted extensive attention for food safety is a worldwide public health problem. Herein, a novel, simple and label-free fluorescent aptasensor, based on quaternized tetraphenylethene salt (TPE-Z), graphene oxide (GO) and AFB1 aptamer, has been constructed to detect AFBI. In the presence of AFB1, AFB1 aptamer undergoes a conformational switch from single stranded structure to the AFB1/AFB1 aptamer complex upon target binding, which induces the release of TPE-Z/AFB1 aptamer from the surface of GO. Thus, the fluorescence of TPE-Z/AFB1 aptamer is recovered. The assay can be performed by simply mixing TPE-Z, AFB1 aptamer, the GO and the AFB1 samples with a detection limit of 0.25 ng/mL. It is highly selective against other aflatoxins in foods and its performance has been verified in food samples (corn, milk and rice) with known concentration AFB1.

【 授权许可】

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