Simultaneous detection of salivary Δ9-tetrahydrocannabinol and alcohol using a Wearable Electrochemical Ring Sensor

TALANTA

卷:211

Article

Mishra, Rupesh K.^1,2  Sempionatto, Juliane R.¹  Li, Zhanhong¹  Brown, Christopher¹  Galdino, Nathalia M.¹  Shah, Rushabh¹  Liu, Shuyang¹  Hubble, Lee J.^1,3  Bagot, Kara^4,5  Tapert, Susan⁴  Wang, Joseph¹ 

[1] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

[2] Amity Univ Rajasthan, Amity Inst Biotechnol, Jaipur 303002, Rajasthan, India

[3] CSIRO Mfg, Lindfield, NSW 2070, Australia

[4] Univ Calif San Diego, Dept Psychiat, La Jolla, CA 92093 USA

[5] Icahn Sch Med Mt Sinai, Addict Inst Mt Sinai, Dept Psychiat, New York, NY 10029 USA

关键词: Wearable THC sensor;    Alcohol sensor;    Marijuana sensor;    Ring sensor;    Saliva sensor;    Electrochemical sensor;   

DOI  :  10.1016/j.talanta.2020.120757

来源: Elsevier

【 摘 要 】

Driving under the influence of cannabis and alcohol represents a major safety concern due to the synergistic or additive effect of these substances of abuse. Hence, rapid road-site testing of these substances is highly desired to reduce risks of fatal accidents. Here we describe a wearable electrochemical sensing device for the simultaneous direct, decentralized, detection of salivary THC and alcohol. The new ring-based sensing platform contains a voltammetric THC sensor and an amperometric alcohol biosensor on the ring cap, along with the wireless electronics embedded within the ring case. Rapid replacement of the disposable sensingelectrode ring cap following each saliva assay is accomplished by aligning spring-loaded pins, mounted on the electronic board (PCB), with the current collectors of the sensing electrodes. The printed dual-analyte sensor ring cover is based on a MWCNT/carbon electrode for the THC detection along with a Prussian-blue transducer, coated with alcohol oxidase/chitosan reagent layer, for the biosensing of alcohol. THC and alcohol can thus be detected simultaneously in the same diluted saliva sample within 3 min, with no cross talk and no interferences from the saliva matrix. The new wearable ring sensor platform should enable law enforcement personnel to screen drivers in a single traffic stop and offers considerable promise for addressing growing concerns of drug-impaired driving.

【 授权许可】

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