Sensitive determination of anions in saliva using capillary electrophoresis after transient isotachophoretic preconcentration

TALANTA

卷:77

Article

Xu, Zhongqi¹  Doi, Takayuki¹  Timerbaev, Andrei R.^2,3  Hirokawa, Takeshi¹ 

[1] Hiroshima Univ, Grad Sch Engn, Dept Appl Chem, Higashihiroshima 7398527, Japan

[2] Russian Acad Sci, Vernadsky Inst Geochem & Analyt Chem, Moscow 119991, Russia

[3] Univ Vienna, Inst Inorgan Chem, A-1090 Vienna, Austria

关键词: Inorganic anions;    Capillary zone electrophoresis;    Preconcentration;    Transient isotachophoresis;    Saliva;   

DOI  :  10.1016/j.talanta.2008.06.017

来源: Elsevier

【 摘 要 】

A transient isotachophoresis-capillary electrophoresis (tITP-CE) system for the determination of minor inorganic anions in saliva is described. The complete separation and quantification of bromide, iodide, nitrate, nitrite, and thiocyanate has been achieved with only centrifugation and dilution of the saliva sample. In-line tITP preconcentration conditions, created by introduction of the plugs of 5 mM dithionic acid (leading electrolyte) and 10 mM formic acid (terminating electrolyte) before and after the sample zone, respectively, allowed the limits of direct UV absorption detection (at 200 nm) to be up to 50-fold improved as compared with CE without tITP As a result, nitrate and thiocyanate were still detectable at 4.6 and 3.8 mu g l(-1), respectively, in 1000 times diluted saliva. The daily variations of anionic concentrations in saliva samples taken from a smoking health volunteer were discussed based on the results of tITP-CE analysis. It was confirmed that the thiocyanate concentration in saliva noticeably increased after smoking. This is apparently the first report on simultaneous quantification of more than four anionic salivary constituents using CE. (c) 2008 Elsevier B.V. All rights reserved.

【 授权许可】

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