Pulsed multi-wavelength excitation using fiber-in-capillary light emitting diode induced fluorescence detection in capillary electrophoresis

TALANTA

卷:83

Article

Huo, Feng^2,3  Yuan, Hongyan^2,3  Yang, Xiupei^4,5  Breadmore, Michael C.¹  Xiao, Dan^2,3 

[1] Univ Tasmania, Australian Ctr Res Separat Sci, Sch Chem, Hobart, Tas, Australia

[2] Sichuan Univ, Coll Chem, Chengdu 610065, Peoples R China

[3] Sichuan Univ, Coll Chem Engn, Chengdu 610065, Peoples R China

[4] China W Normal Univ, Coll Chem, Nanchong 637002, Peoples R China

[5] China W Normal Univ, Coll Chem Engn, Nanchong 637002, Peoples R China

关键词: Multi-wavelength;    Pulsed LED;    Gradient index (GRIN) lens;    Collimation;    Fluorescence detection;    Capillary electrophoresis;   

DOI  :  10.1016/j.talanta.2010.09.047

来源: Elsevier

【 摘 要 】

A novel instrument was developed using a multi-wavelength pulsed LED array with in-column optic-fiber induced fluorescence detection by capillary electrophoresis. The light from 2 different wavelength LEDs (450 nm and 480 nm) was pulsed for short intervals at high intensity. The beam from each LED was collimated and reshaped with the gradient index (GRIN) lens group to achieve a highly effective coupling between LED light source and an optical fiber. The optical fiber was placed inside the capillary for in-capillary LED-induced fluorescence detection. The advantages of this system were validated by the simultaneous determination of vitamin B2 and fluorescein. Detection limits for vitamin B2 and fluorescein were estimated to be 5 nM and 0.29 nM (S/N = 3), respectively. The relative standard deviations (RSDs, n = 6) of the both compounds for migration time and peak area were better than 0.83%. 2.20% and 1.21%, 2.75%, respectively. The method was applied to the determination of vitamin B2 in commercial tablets and fluorescein in fluorescein sodium injection and the recoveries obtained were in the range of 96.6-102.0% and 99.9-102.8%, respectively. It was also applied to human serum, where the recoveries were found to be in the range of 94.4-97.0% and 92.6-96.4%, respectively. The system has been successfully applied in separation and determination of the both biological samples with acceptable analytical performance. (C) 2010 Elsevier B.V. All rights reserved.

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