Biomimetic magnetic sensor for electrochemical determination of scombrotoxin in fish

TALANTA

卷:194

Article

Hassan, Amal H. A.^1,2  Sappia, Luciano¹  Lima Moura, Silio¹  Ali, Fatma H. M.²  Moselhy, Walaa A.³  Taboada Sotomayor, Maria del Pilar⁴  Isabel Pividori, Maria¹ 

[1] Univ Autonoma Barcelona, Dept Quim, Grp Sensors & Biosensors, Bellaterra 08193, Spain

[2] Beni Suef Univ, Fac Vet Med, Food Hyg & Control Dept, Bani Suwayf 62511, Egypt

[3] Beni Suef Univ, Fac Vet Med, Forens Med & Toxicol Dept, Bani Suwayf 62511, Egypt

[4] State Univ Sao Paulo UNESP, Inst Chem, Dept Analyt Chem, BR-14801970 Araraquara, SP, Brazil

关键词: Magnetic-MIP;    Scombrotoxin;    Histamine;    Electrochemical determination;    Fish;    Food safety;   

DOI  :  10.1016/j.talanta.2018.10.066

来源: Elsevier

【 摘 要 】

This work addresses a novel, rapid and cost-effective approach for the electrochemical sensing of scombrotoxin (histamine) in fish based on magnetic molecularly imprinted polymer (magnetic-MIP). The histamine magnetic-MIP was synthesized by the core-shell method using histamine as a template, and 2-vinyl pyridine as functional monomer. The magnetic-MIP was characterized by TEM, SEM, and confocal microscopy. Additionally, the binding capacity of magnetic-MIP towards histamine was investigated and compared with magnetic non-molecularly imprinted polymer (magnetic-NIP). This biomimetic material merged the advantages of MIPs and magnetic particles (MPs), including low cost of production, stability, high binding capacity and can be easily separated by the aid of a permanent magnet. The magnetic-MIP was integrated into magneto-actuated electrodes for the direct electrochemical detection of histamine preconcentrated from fish samples. The results revealed that this approach succeeded in the preconcentration and determination of histamine with a LOD as low as 1.6 x 10(-6) mg L-1, much lower than the index for fish spoilage (50 mg kg(-1)) accordingly to the legislation. Furthermore, the analytical performance was validated for the determination of histamine in scombroid fish samples with recovery values ranging from 96.8 to 102.0 %, confirm so it can be applied easily for routine food examination.

【 授权许可】

Free