Determination of 9, 10-phenanthrenequinone in airborne particulates by high-performance liquid chromatography with post-column fluorescence derivatization using 2-aminothiophenol

TALANTA

卷:81

Article

Kishikawa, Naoya¹  Nakashima, Hajime¹  Ohyama, Kaname¹  Nakashima, Kenichiro¹  Kuroda, Naotaka¹ 

[1] Nagasaki Univ, Grad Sch Biomed Sci, Course Pharmaceut Sci, Nagasaki 8528521, Japan

关键词: Phenanthrenequinone;    Fluorogenic derivatization;    Airborne particulates;   

DOI  :  10.1016/j.talanta.2010.03.053

来源: Elsevier

【 摘 要 】

9, 10-Phenanthrenequinone (PQ) is regarded as a harmful environmental pollutant and its presence has been reported in atmospheric environment. The measurement of PQ in environment should be necessary to evaluate the influence of PQ on human health We found that PQ reacted with 2-aminothiophenol under acidic condition to form fluorescent derivative which emits green fluorescence at 510 nm. Based on this reaction, a simple and rapid determination method for PQ was developed by HPLC with post-column derivatization and fluorescence detection By the proposed HPLC system, PQ was detected at 24 min and the detection limit was 67 fmol/injection (S/N = 3). The proposed method was able to determine the atmospheric PQ concentrations by the direct injection of extract from airborne particulates Crown Copyright (C) 2010 Published by Elsevier B V All rights reserved

【 授权许可】

Free