Quinoline-based ratiometric fluorescent probe for detection of physiological pH changes in aqueous solution and living cells

TALANTA

卷:192

Article

Liu, Zhen¹  Li, Guoping¹  Wang, Yana¹  Li, Jiulong¹  Mi, Yang²  Zou, Dapeng¹  Li, Tiesheng¹  Wu, Yangjie¹ 

[1] Zhengzhou Univ, Coll Chem & Mol Engn, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Sch Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China

关键词: Fluorescent probe;    pH sensing;    Ratiometric response;    Bioimaging;   

DOI  :  10.1016/j.talanta.2018.09.026

来源: Elsevier

【 摘 要 】

Herein, a novel quinoline-based fluorescent probe DQPH has been developed for ratiometric detection of subtle pH fluctuation in biosystem. Upon altering the pH from 4.50 to 9.00, the emission spectra exhibit a large hypsochromic shift (57 nm) and the ratio of fluorescence intensity (F-531 nm/F-588 nm) changes from 0.30 to 1.36 with an ideal pK(a) value of 7.18 and a linear pH variation range of 6.35-8.00. The ratiometric response is attributed to the protonation-activable resonance charge transfer (PARCT) process, which has been proved by H-1 NMR and NOESY experiment. This probe displayed good solubility, low cytotoxicity, anti-interference capability and reversible pH sensing. Furthermore, DQPH was successfully applied for monitoring pH changes in living cells.

【 授权许可】

Free