Surface-specific washing-free immunosensor for time-resolved cortisol monitoring

TALANTA

卷:225

Article

Safarian, Sofia M.¹  Kusov, Pavel A.¹  Kosolobov, Sergey S.¹  Borzenkova, Oksana, V¹  Khakimov, Artem, V¹  Kotelevtsev, Yuri, V¹  Drachev, Vladimir P.^1,2 

[1] Skolkovo Inst Sci & Technol, 3 Nobel St, Moscow 143025, Russia

[2] Univ North Texas, Denton, TX 76203 USA

关键词: Competitive immunoassay;    Metal enhanced fluorescence;    Cortisol;    Real-time measurement;    Gold nanostructures;    BSA-cortisol conjugate;   

DOI  :  10.1016/j.talanta.2020.122070

来源: Elsevier

【 摘 要 】

Cortisol is a steroid hormone that regulates a wide range of vital processes. Its level changes with diurnal rhythm and reacts to stress. Measurement of cortisol levels is still a complex multi-step process. A reversible washing-free registration method is required. Here we describe metal-enhanced fluorescence assay based on a displacement of a dye labeled BSA-cortisol conjugate from the immune complex immobilized on the golden islands by free cortisol. This competitive approach allows time-resolved monitoring of the fluorescent signal, surface-enhanced by the gold film, and provides the possibility of continuous real-time cortisol monitoring based on the implantable surface-enhanced immunosensor, which was not demonstrated so far even in vitro.

【 授权许可】

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