Quantification of PCDD/Fs and dioxin-like PCBs in small amounts of human serum using the sensitive H1L7.5c1 mouse hepatoma cell line: Optimization and analysis of human serum samples from adolescents of the Flemish human biomonitoring program FLEHS II

TALANTA

卷:85

Article

Croes, K.^1,8  Van Langenhove, K.¹  Den Hond, E.²  Bruckers, L.³  Colles, A.²  Koppen, G.²  Loots, I.⁴  Nelen, V.⁵  Schoeters, G.²  Nawrot, T.^6,7  Van Larebeke, N.⁸  Denison, M. S.⁹  Vandermarken, T.¹  Elskens, M.¹  Baeyens, W.¹ 

[1] Vrije Univ Brussel, Dept Analyt & Environm Chem ANCH, B-1050 Brussels, Belgium

[2] Flemish Inst Technol Res Vito Environm Hlth & Res, Mol, Belgium

[3] Hasselt Univ, Interuniv Inst Biostat & Stat Bioinformat, Diepenbeek, Belgium

[4] Univ Antwerp, B-2020 Antwerp, Belgium

[5] Prov Inst Hyg, Antwerp, Belgium

[6] Katholieke Univ Leuven, Unit Lung Toxicol, Louvain, Belgium

[7] Hasselt Univ, Ctr Environm Sci, Diepenbeek, Belgium

[8] Ghent Univ Hosp, Study Ctr Carcinogenesis & Primary Prevent Canc, B-9000 Ghent, Belgium

[9] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA

关键词: PCDD/Fs;    Dioxin-like PCBs;    CALUX;    Human serum;    Biomonitoring;    FLEHS II;   

DOI  :  10.1016/j.talanta.2011.07.103

来源: Elsevier

【 摘 要 】

Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast and inexpensive tool for the throughput analysis of dioxin-like compounds in a large number of samples and requires only small sample volumes, the use of this technique in human biomonitoring programs provides a good alternative to GC-HRMS. In this study, a method for the separate analysis of PCDD/Fs and dioxin-like PCBs (dl-PCBs) in human serum with the new sensitive H1L7.5c1 mouse hepatoma cell line was optimized. Sample dilution factors of 5 and 2.4 were selected for routine analysis of respectively the PCDD/Fs and dl-PCBs. The validation studies showed that repeatability and within-lab reproducibility for the quality control (QC) standard were within the in-house criteria. A long-term within-lab reproducibility of 25% for the PCDD/F fraction and 41% for the dl-PCB fraction for the analysis of pooled serum samples, expressed as pg BEQ/g fat, was determined. CALUX recoveries of the spiked procedural blanks were within the acceptable in-house limits of 80-120% for both fractions and the LOQ was 30.3 pg BEQ/g fat for the PCDD/Fs and 14.5 pg BEQ/g fat for the dl-PCBs. The GC-HRMS recovery of a C13-spiked pooled serum sample was between 60 and 90% for all PCDD/F congeners and between 67 and 82% for the non-ortho PCBs. An adequate separation between both fractions was found. The CALUX/GC-HRMS ratio for a pooled serum sample was respectively 2.0 and 1.4 for the PCDD/Fs and the dl-PCBs, indicating the presence of additional AhR active compounds. As expected, a correlation was found between human serum samples analyzed with both the new H1L7.5c1 cell line and the more established H1L6.1c3 cell line. The geometric mean CALUX-BEQ values, reported for the adolescents of the second Flemish Environment and Health Study (FLEHS II) recruited in 2009-2010, were 108(95% CI: 101-114) pg CALUX-BEQ/g fat for the PCDD/Fs and 32.1 (30.1-34.2) pg CALUX-BEQ/g fat for the dioxin-like PCBs. 2011 Elsevier By. All rights reserved.

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