2-picolylamine derivatization for high sensitivity detection of abscisic acid in apicomplexan blood-infecting parasites

TALANTA

卷:168

Article

Moles, Ernest^1,2,3  Marcos, Josep^4,5  Imperial, Santiago^3,6  Pozo, Oscar J.⁴  Fernandez-Busquets, Xavier^1,2,3 

[1] Inst Bioengn Catalonia IBEC, Nanomalaria Grp, Baldiri Rebcac 10-12, ES-08028 Barcelona, Spain

[2] Univ Barcelona, Hosp Clin, CRESIB, Barcelona Ctr Int Hlth Res,Barcelona Inst Global, Rossello 149-153, ES-08036 Barcelona, Spain

[3] Univ Barcelona, Nanosci & Nanotechnol Inst IN2UB, Marti & Frangues 1, ES-08028 Barcelona, Spain

[4] Hosp Mar, IMIM, Bioanal Res Grp, Doctor Aiguader 88, ES-08003 Barcelona, Spain

[5] Univ Pompeu Fabra, Dept Expt & Hlth Sci, Doctor Aiguader 88, ES-08003 Barcelona, Spain

[6] Univ Barcelona, Dept Biochem & Mol Biomed, Avda Diagonal 643, ES-08028 Barcelona, Spain

关键词: Liquid chromatography-electrospray;    ionization tandem mass spectrometry;    Abscisic acid;    Picolylamine;    Malaria;    Plasmodium falciparum;    Apicomplexa;   

DOI  :  10.1016/j.talanta.2017.03.030

来源: Elsevier

【 摘 要 】

We have developed a new liquid chromatography-electrospray ionization tandem mass spectrometry methodology based on 2-picolylamine derivatization and positive ion mode detection for abscisic acid (ABA) identification. The selected reaction leads to the formation of an amide derivative which contains a highly active pyridyl group. The enhanced ionization allows for a 700-fold increase over commonly monitored unmodified ABA, which in turn leads to excellent limits of detection and quantification values of 0.03 and 0.15 ng mL(-1), respectively. This method has been validated in the highly complex matrix of a red blood cell extract. In spite of the high sensitivity achieved, ABA could not be detected in Plasmodium falciparum-infected red blood cells, suggesting that, if present, it will be found either in ultratrace amounts or as brief bursts at defined time points within the intraerythrocytic cycle and/or in the form of a biosynthetic analogue.

【 授权许可】

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