Development of a SERS strategy to overcome the nanoparticle stabilisation effect in serum-containing samples: Application to the quantification of dopamine in the culture medium of PC-12 cells

TALANTA

卷:186

Article

Dumont, E.¹  De Bleye, C.¹  Cailletaud, J.¹  Sacre, P. -Y.¹  Van Lerberghe, P. -B.²  Rogister, B.^3,4  Rance, G. A.⁵  Aylott, J. W.⁶  Hubert, Ph.¹  Ziemons, E.¹ 

[1] Univ Liege ULiege, Dept Pharm, Lab Pharmaceut Analyt Chem, VibraSante Hub,CIRM,CHU, B36, B-4000 Liege, Belgium

[2] Univ Liege ULiege, Lab Dev Neurobiol, GIGA Neurosci, CHU, B36, B-4000 Liege, Belgium

[3] Univ Liege ULiege, Lab Nervous Syst Disorders & Therapy, GIGA Neurosci, CHU, B36, B-4000 Liege, Belgium

[4] CHU Liege, Dept Neurol, B-4000 Liege, Belgium

[5] Univ Nottingham, Nanoscale & Microscale Res Ctr, Univ Pk, Nottingham NG7 2RD, England

[6] Univ Nottingham, Sch Pharm, Lab Biophys & Surface Anal, Univ Pk, Nottingham NG7 2RD, England

关键词: Surface-enhanced Raman scattering (SERS);    Gold nanoparticles;    Dopamine;    Quantitative methods;    Cellular analysis;    PC-12 cells;   

DOI  :  10.1016/j.talanta.2018.04.038

来源: Elsevier

【 摘 要 】

The analysis of serum samples by surface-enhanced Raman spectroscopy (SERS) has gained ground over the last few years. However, the stabilisation of colloids by the proteins contained in these samples has restricted their use in common practice, unless antibodies or aptamers are used. Therefore, this work was dedicated to the development of a SERS methodology allowing the analysis of serum samples in a simple and easy-to-implement way. This approach was based on the pre-aggregation of the colloid with a salt solution. Gold nanoparticles (AuNPs) were used as the SERS substrate and, owing to its physiopathological importance, dopamine was chosen as a model to implement the SERS approach. The presence of this neurotransmitter could be determined in the concentration range 0.5-50 ppm (2.64-264 mu M) in the culture medium of PC-12 cells, with a R-2 of 0.9874, and at even lower concentrations (0.25 ppm, 1.32 mu M) in another matrix containing fewer proteins. Moreover, the effect of calcium and potassium on the dopamine exocytosis from PC-12 cells was studied. Calcium was shown to have a predominant and dose-dependant effect. Finally, PC-12 cells were exposed to dexamethasone in order to increase their biosynthesis and release of dopamine. This increase was monitored with the developed SEAS approach.

【 授权许可】

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