Statistical mixture design development of digestion methods for Oyster tissue using inductively coupled plasma optical emission spectrometry for the determination of metallic ions

TALANTA

卷:80

Article

Nano, R. M. W.^1,2  Bruns, R. E.¹  Ferreira, S. L. C.³  Baccan, N.^1,4  Cadore, S.¹ 

[1] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP, Brazil

[2] Ctr Fed Educ Tecnol, BR-40301015 Salvador, BA, Brazil

[3] Univ Fed Bahia, Inst Quim, BR-40170290 Salvador, BA, Brazil

[4] Fac Paulinia, BR-13140000 Paulinia, SP, Brazil

关键词: Statistical mixture design;    Simplex centroid design;    Biological samples;    Metallic ions;    Atomic emission spectrometry;   

DOI  :  10.1016/j.talanta.2009.07.025

来源: Elsevier

【 摘 要 】

The quantitative determination of chemical elements in organic or biological samples is an important analytical problem. Normally the elements to be determined in the organic matrix must be transformed into a simple inorganic form. A digestion method by heating on a block digestor has been developed for the determination of Al, As, Ba, Ca, Cd, Co, Cu, Fe, Mg, Mn, V and Zn in Oyster tissue by ICP OES. A simplex centroid statistical mixture design has been used to study the effects of changing HNO3, HCll and H2O2 reagent proportions on the digestion of these samples. Response surface and principal component analyses show that the species Ca, Cd, Cu, Fe, Mg, Mn and Zn have very similar analytical tendencies under this experiment. By means of mixture modeling maximum recoveries for these ions were predicted using 19%,18% and 63% of the HCl, HNO3 and H2O2 pseuclocomponent mixtures, respectively. This corresponds to 21.4%,30.8% and 47.8% of the HCl, HNO3 and H2O2 commercial solutions. Furthermore the As, Co and V ions present large recoveries for these mixtures as well. The Al and Ba ion recoveries are seen to be independent of the mixture proportions. The analysis of Oyster tissue reference material (SRM 1566b - NIST) under optimized conditions at the selected wavelengths resulted in ion recoveries between 90% and 100%. (C) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

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