Characterisation of biosynthesised silver nanoparticles by scanning electrochemical microscopy (SECM) and voltammetry

TALANTA

卷:132

Article

Battistel, Dario¹  Baldi, Franco²  Gallo, Michele²  Faleri, Claudia³  Daniele, Salvatore² 

[1] Univ Ca Foscari Venice, Dipartimento Sci Ambientali Infomat & Stat, I-30127 Venice, Italy

[2] Univ Ca Foscari Venice, Dipartimento Sci Mol & Nanosistemi, I-30127 Venice, Italy

[3] Univ Siena, Dipartimento Sci Vita, I-53100 Siena, Italy

关键词: Silver nanoparticles;    Bacteria;    Biosynthesis;    Scanning electrochemical microscopy;    Anodic stripping voltammetry;   

DOI  :  10.1016/j.talanta.2014.09.023

来源: Elsevier

【 摘 要 】

Silver nanoparticles (AgNPs) were biosynthesised by a Klebsiella oxytoca strain BAS-10, which, during its growth, is known to produce a branched exopolysaccharide (EPS). Klebsiella oxytoca cultures, treated with AgNO3 and grown under either aerobic or anaerobic conditions, produced silver nanoparticles embedded in EPS (AgNPs-EPS) containing different amounts of Ag(0) and Ag(I) forms. The average size of the AgNPs-EPS was determined by transmission electron microscopy, while the relative abundance of Ag (0)- or Ag(I)-containing AgNPs-EPS was established by scanning electrochemical microscopy (SECM). Moreover, the release of silver(I) species from the various types of AgNPs-EPS was investigated by combining SECM with anodic stripping voltammetry. These measurements allowed obtaining information on the kinetic of silver ions release from AgNPs-EPS and their concentration profiles at the substrate/water interface. (C) 2014 Elsevier B.V. All rights reserved.

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