Rapid micromotor-based naked-eye immunoassay

TALANTA

卷:167

Article

de Avila, Berta Esteban-Fernandez¹  Zhao, Mingjiao^1,2  Campuzano, Susana³  Ricci, Francesco²  Pingarron, Jose M.³  Mascini, Marcello^1,3,4  Wang, Joseph¹ 

[1] Univ Calif, Dept Nanoengn, San Diego, CA 92093 USA

[2] Univ Rome, Dept Chem, Via Ric Sci, I-00133 Rome, Italy

[3] Univ Complutense Madrid, Dept Analyt Chem, E-28040 Madrid, Spain

[4] Univ Teramo, Fac Biosci & Technol Food Agr & Environm, I-64100 Teramo, Italy

关键词: Cortisol;    Real-time sensing;    Micromotor;    Antibody;    Naked-eye detection;   

DOI  :  10.1016/j.talanta.2017.02.068

来源: Elsevier

【 摘 要 】

A dynamic micromotor-based immunoassay, exemplified by cortisol detection, based on the use of tubular micromotors functionalized with a specific antibody is described. The use of antibody-functionalized micro motors offers huge acceleration of both direct and competitive cortisol immunoassays, along with greatly enhanced sensitivity of direct and competitive immunoassays. The dramatically improved speed and sensitivity reflect the greatly increased likelihood of antibody-cortisol contacts and fluid mixing associated with the dynamic movement of these microtube motors and corresponding bubble generation that lead to a highly efficient and rapid recognition process. Rapid naked-eye detection of cortisol in the sample is achieved in connection to use of horseradish peroxidase (HRP) tag and TMB/H2O2 system. Key parameters of the competitive immunoassay (e.g., incubation time and reaction volume) were optimized. This fast visual micromotor-based sensing approach enables on the move specific detection of the target cortisol down to 0.1 mu g mL(-1) in just 2 min, using ultrasmall (50 mu L) sample volumes.

【 授权许可】

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