Stand out from matrix: Ultra-sensitive LC-MS/MS method for determination of histamine in complex biological samples using derivatization and solid phase extraction

TALANTA

卷:225

Article

Hu, Xiaolan¹  Bian, Xiqing¹  Gu, Wan-Yi¹  Sun, Baoqing²  Gao, Xiang³  Wu, Jian-Lin¹  Li, Na¹ 

[1] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Ave Wai Long, Taipa, Macao, Peoples R China

[2] Guangzhou Med Univ, Natl Clin Ctr Resp Dis, Guangzhou Inst Resp Dis, State Key Lab Resp Dis,Affiliated Hosp 1, Guangzhou, Peoples R China

[3] Xiamen Univ, Sch Pharmaceut Sci, Xiamen 361102, Fujian, Peoples R China

关键词: Histamine;    Diisopropyl phosphite-derivatization;    Solid phase extraction;    LC-MS/MS quantification;    Asthma;    Mast cell;   

DOI  :  10.1016/j.talanta.2020.122056

来源: Elsevier

【 摘 要 】

The determination of low abundant endogenous components is a challenge for the clinical samples. Histamine, a crucial endogenous component, fulfils various regulatory and mediatory functions in human, and the change of content is a critical index for the diagnosis of some diseases, especially allergy, asthma, and anaphylactic shock. However, it is challenging to detect histamine because of the low stability and concentration in complex biological samples. Here we developed an ultra-sensitive and accurate LC-MS/MS quantification method based on derivatization, isotope dilution, and solid phase extraction. The derivatization of histamine with diisopropyl phosphite (DIPP) not only enhanced the retention on the LC column but also improved the ionization efficiency. Next, solid phase extraction was applied to remove the interference, which finally resulted in standing out of the trace histamine from the high contents of the matrix. The lowest limit of quantification (LLOQ) was 0.1 pg/mL that is enough low to determine the histamine in one cell and low nano-liter of serum. This approach was successfully applied for the quantification of histamine in clinical serum samples of asthma patients and mast cell treated with chemicals modulating histamine release.

【 授权许可】

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