Development of a new analytical tool for assessing the mutagen 2-methyl-1, 4-dinitro-pyrrole in meat products by LC-ESI-MS/MS

TALANTA

卷:185

Article

Molognoni, Luciano^1,2,3  Daguer, Heitor¹  de Sa Ploencio, Leandro Antunes^1,4  Yotsuyanagi, Suzana Eri⁵  da Silva Correa Lemos, Ana Lucia⁵  Joussef, Antonio Carlos⁴  Lindner, Juliano De Dea³ 

[1] Minist Agr Pecuaria & Abastecimento, Lab Nacl Agr, Secao Lab Avancada Sao Jose, SLAV SC LANAGRO RS, BR-88102600 Sao Jose, SC, Brazil

[2] Inst Catarinense Sanidade Agr ICASA, BR-88034000 Florianopolis, SC, Brazil

[3] Univ Fed Santa Catarina, Ctr Ciencias Agr, Dept Ciencia & Tecnol Alimentos, BR-88034000 Florianopolis, SC, Brazil

[4] Univ Fed Santa Catarina, Ctr Ciencias Fis & Matemat, Dept Quim, BR-88040900 Florianopolis, SC, Brazil

[5] Secretaria Agr & Abastecimento Estado Sao Paulo, Inst Tecnol Alimentos, Ctr Tecnol Carnes, BR-13073001 Campinas, SP, Brazil

关键词: Analytical validation;    Commission Decision 2002/657/EC;    DNMP;    Food safety;    Food preservatives;    Measurement uncertainty;   

DOI  :  10.1016/j.talanta.2018.03.035

来源: Elsevier

【 摘 要 】

The use of sorbate and nitrite in meat processing may lead to the formation of 2-methyl-1,4-dinitro-pyrrole (DNMP), a mutagenic compound. This work was aimed at developing and validating an analytical method for the quantitation of DNMP by liquid chromatography-tandem mass spectrometry. Full validation was performed in accordance to Commission Decision 2002/657/EC and method applicability was checked in several samples of meat products. A simple procedure, with low temperature partitioning solid-liquid extraction, was developed. The nitrosation during the extraction was monitored by the N-nitroso-DL-pipecolic acid content. Chromatographic separation was achieved in 8 min with di-isopropyl-3-aminopropyl silane bound to hydroxylated silica as stationary phase. Samples of bacon and cooked sausage yielded the highest concentrations of DNMP (68 +/- 3 and 50 +/- 3 mu g kg(-1), respectively). The developed method proved to be a reliable, selective, and sensitive tool for DNMP measurements in meat products.

【 授权许可】

Free