Simultaneous determination of first-line anti-tuberculosis drugs by capillary zone electrophoresis using direct UV detection

TALANTA

卷:82

Article

Faria, Adriana F.¹  de Souza, Marcus V. N.²  Bruns, Roy E.³  de Oliveira, Marcone A. L.¹ 

[1] Univ Fed Juiz Fora, Dept Quim, Inst Ciencias Exatas, BR-36036330 Juiz De Fora, MG, Brazil

[2] Fundacao Oswaldo Cruz, Inst Tecnol Farmacos Far Manguinhos, BR-21041250 Rio De Janeiro, Brazil

[3] Univ Estadual Campinas, Inst Quim, Dept Fisicoquim, BR-13083970 Campinas, SP, Brazil

关键词: Capillary zone electrophoresis;    Ethambutol;    Isoniazid;    Rifampicin;    Pyrazinamide;    Pharmaceutical formulation;   

DOI  :  10.1016/j.talanta.2010.04.044

来源: Elsevier

【 摘 要 】

An alternative methodology for simultaneous analysis of ethambutol, isoniazid, rifampicin and pyrazinamide in pharmaceutical formulations by capillary zone electrophoresis under UV direct detection with an analysis time of 8.0 min is proposed. Background running was based on the effective mobility curve of the analytes and an optimum separation condition was achieved using a 3(3) Box-Behnken design, with Brij 35, Cu2+ and acetic acid/sodium acetate buffer as factors. An electrolyte consisting of 50.0 mmol L-1 of acetic acid/sodium acetate buffer:12.5 mmol L-1 of CuSO4, and standard and sample solutions prepared in 2.00 mmol L-1 of Brij 35 and 12.5 mmol L-1 of CuSO4 were optimized. After evaluating validation parameters, the method was successfully applied to the analysis of samples in the form of tablets and sachets. (C) 2010 Elsevier By. All rights reserved.

【 授权许可】

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