Fe3O4@Au nanoparticles-based magnetoplatform for the HMGA maize endogenous gene electrochemical genosensing

TALANTA

卷:206

Article

Sousa, Juliana Beatriz^1,2  Ramos-Jesus, Joilson³  Silva, L. C.⁴  Pereira, C.⁵  de-los-Santos-Alvarez, N.⁶  Fonseca, Rosana A. S.⁴  Miranda-Castro, R.⁶  Delerue-Matos, C.²  Ribeiro Santos Junior, J.¹  Fatima Barroso, M.² 

[1] Univ Fed Piaui UFPI, Programa Posgrad Biotecnol RENORBIO Proreitoria P, Teresina, Brazil

[2] Inst Politecn Porto, Inst Super Engn Porto, REQUIMTE LAQV, Porto, Portugal

[3] UFPI, Nucleo Pesquisa Biodiversidade & Biotecnol, Parnaiba, Brazil

[4] Univ Pernambuco, ICB, Recife, PE, Brazil

[5] Univ Porto, Fac Ciencias, Dept Quim & Bioquim, REQUIMTE LAQV, Porto, Portugal

[6] Univ Oviedo, Dept Quim Fis & Analit, Oviedo, Spain

关键词: Fe3O4@Au magnetic nanoparticles;    Electrochemical genosensors;    GMO;    Maize;    HMGA gene;   

DOI  :  10.1016/j.talanta.2019.120220

来源: Elsevier

【 摘 要 】

This work addresses a technological advance applied to the construction of a magnetogenoassay with electrochemical transduction for the maize taxon-specific (HMGA gene) detection using gold-coated magnetic nanoparticles as nanosized platform. Superparamagnetic core-shell Fe3O4@Au nanoparticles (10.4 +/- 1.7 nm) were used to assemble the genoassay through the covalent immobilization of HMGA DNA probes onto carboxylated self-assembled monolayers at the nanoparticles surface. A hybridization reaction using sandwich format was selected to prevent inefficient hybridization connected with stable secondary DNA structures using also fluorescein isothiocyanate as DNA signaling tag. The labelling of the hybridization reaction with enzymes allowed the chronoamperometric measurement of the peroxidase activity linked to the nanoplatform located on gold surface. Using this electrochemical magnetogenoassay a linear concentration range from 0.5 to 5 nM and a LOD of 90 pM with a RSD <1.2% was calculated. Certified maize was evaluated without further purification after PCR amplification. This work highlights the efficacy of the electrochemical magnetogenoassay for the HMGA detection, showing its potential as alternative procedure for the verification of the compliance of the legislation.

【 授权许可】

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