Capillary zone electrophoresis method to assay tipranavir capsules and identification of oxidation product and organic impurity by quadrupole-time of flight mass spectrometry

TALANTA

卷:181

Article

Lago, Matheus Wagner¹  Friedrich, Mariane Lago²  Iop, Gabrielle Dineck³  de Souza, Thiago Belarmino²  Meho, Paola de Azevedo³  Horn Adams, Andrea Ines² 

[1] Univ Fed Santa Maria, Programa Posgrad Ciencias Farmaceut, Santa Maria, RS, Brazil

[2] Univ Fed Santa Maria, Dept Farm Ind, Santa Maria, RS, Brazil

[3] Univ Fed Santa Maria, Dept Quim, Santa Maria, RS, Brazil

关键词: Tipranavir;    Stability;    Degradation products;    Oxidation;    Capillary zone electrophoresis;    High resolution mass spectrometry;   

DOI  :  10.1016/j.talanta.2018.01.012

来源: Elsevier

【 摘 要 】

Tipranavir (TPV) is one of the most recently developed protease inhibitors (PI) and it is specially recommended for treatment-experienced patients who are resistant to other PI drugs. In this work, a simple and friendly environmental CZE stability-indicating method to assay TPV capsules was developed and two TPV organic impurities were identified by high resolution mass spectrometry (HRMS). The optimized analytical conditions were: background electrolyte composed of sodium borate 50 mM, pH 9.0 and 5% of methanol; voltage + 28 kV; hydrodynamic injection of 5 s (100 mbar), detection wavelength 240 nm, at 25 degrees C. The separation was achieved in a fused silica capillary with 50 mu m x 40 cm (inner diameter x effective length), using furosemide as internal standard. All the validation parameters were met and the method was specific, even in the presence of degradation products and impurities. Oxidation was indicated as the main degradation pathway among those evaluated in this study (acidic, alkaline, thermal, photolytic and oxidative) and it showed a second order degradation kinetic, under the conditions used in this study. The main oxidation product and an organic impurity detected in the standard were characterized by Q-TOF, and both of them correspond to oxidation products of TPV.

【 授权许可】

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