Enantio selective detection of chiral phosphorescent analytes in cyclodextrin complexes

TALANTA

卷:66

Article

García-Ruiz, C ; Scholtes, MJ ; Ariese, F ; Gooijer, C

关键词: camphorquinone;    inclusion complex;    room temperature phosphorescence;    phosphorescence lifetime;   

DOI  :  10.1016/j.talanta.2004.12.010

来源: Elsevier

【 摘 要 】

Inclusion complexes between camphorquinone (CQ) and cyclodextrins (CDs) in deoxygenated aqueous solutions are shown to exhibit relatively strong room temperature phosphorescence (RTP). Among the various CDs tested, alpha-CD showed the strongest RTP signals. Interestingly, these signals differed significantly for the two enantiomers of CQ; the phosphorescence lifetime of (+)-CQ was about four times longer than that of (-)-CQ, being 352 +/- 16 and 89 +/- 6 mu s, respectively. This enantiomeric selectivity is attributed to a difference in dissociation rates (competing with the radiative emission process) for the diastereoisomeric inclusion complexes dealt with, which have a 2:1 stoichiometry (alpha-CD:CQ:alpha-CD). Time-resolved RTP detection using different delay times enables the determination of the two enantiomers in a mixture without involving a separation technique. The minimum detectable fraction of (+)-CQ in a 2 mM sample was 13%. (c) 2004 Elsevier B.V. All rights reserved.

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