Investigation of midazolam electro-oxidation on boron doped diamond electrode by voltammetric techniques and density functional theory calculations: Application in beverage samples

TALANTA

卷:207

Article

Rocha, Raquel G.¹  Silva, Weberson P.¹  Sousa, Raquel M. F.¹  Junior, Moacyr C.¹  Santana, Mario H. P.²  Munoz, Rodrigo A. A.¹  Richter, Eduardo M.¹ 

[1] Univ Fed Uberlandia, Inst Chem, BR-38400092 Uberlandia, MG, Brazil

[2] Reg Superintendence Brazilian Fed Police Minas Ge, Tech & Sci Unit, BR-38408680 Uberlandia, MG, Brazil

关键词: Selectivity;    Electrochemical mechanism;    Forensic chemistry;    On-site analysis;    Hypnotic-sedative drug;   

DOI  :  10.1016/j.talanta.2019.120319

来源: Elsevier

【 摘 要 】

Midazolam (MID) is a sedative drug which can be added in beverage samples as drug-facilitated-sexual assault (date rape drug). This type of drug has short half-life in biological fluids (not detectable) which often prevents the correlation between drug abuse and crime. In this work, we described a simple and low-cost method for fast screening and selective determination of MID in beverage samples (vodka, whiskey and red wine). For the first time, the electrochemical oxidation of MID was used for this purpose. The oxidation mechanism was studied using electrochemical techniques (cyclic and square-wave voltammetry) and computational simulations (density functional theory calculations). Differential-pulse voltammetry, boron-doped diamond electrode (BDDE), and Britton-Robinson (BR) buffer (pH = 2) were selected as electrochemical analysis technique, working electrode and supporting electrolyte, respectively. Different linear response ranges (4-25 mu mol L-1 with r = 0.9972; 1-10 mu mol L-1 with r = 0.9951; 1-15 mu mol L-1 with r = 0.9982) and limits of detection (0.46, 0.43 and 0.33 mu mol L-1) were obtained for the analysis of vodka, whisky, and red wine solutions, respectively. The precision and accuracy were satisfactory considering the low relative standard deviation values (RSD < 6.3%, n = 15) and minimal sample matrix effects (recovery values between 87 and 103%).

【 授权许可】

Free