Quantification of guanosine triphosphate and tetraphosphate in plants and algae using stable isotope-labelled internal standards

TALANTA

卷:219

Article

Bartoli, Julia¹  Citerne, Sylvie²  Mouille, Gregory²  Bouveret, Emmanuelle³  Field, Ben⁴ 

[1] Aix Marseille Univ, CNRS, LISM, UMR 7255,IMM FR 3479, 31 Chemin Joseph Aiguier, F-13009 Marseille, France

[2] Univ Paris Saclay, AgroParisTech, INRAE, Inst Jean Pierre Bourgin, F-78000 Versailles, France

[3] Inst Pasteur, Dept Microbiol, Stress Adaptat & Metab Enterobacteriae Unit, F-75015 Paris, France

[4] Aix Marseille Univ, CEA, CNRS, UMR 7265,BIAM, F-13009 Marseille, France

关键词: Guanosine tetraphosphate;    Bacteria;    Plants;    Algae;    Stable isotope labelling;    LC-MS/MS;   

DOI  :  10.1016/j.talanta.2020.121261

来源: Elsevier

【 摘 要 】

Guanosine tetraphosphate (G4P) and guanosine pentaphosphate (G5P) are signalling nucleotides found in bacteria and photosynthetic eukaryotes that are implicated in a wide-range of processes including stress acclimation, developmental transitions and growth control. Measurements of G4P/G5P levels are essential for studying the diverse roles of these nucleotides. However, G4P/G5P quantification is particularly challenging in plants and algae due to lower cellular concentrations, compartmentalization and high metabolic complexity. Despite recent advances the speed and accuracy of G4P quantification in plants and algae can still be improved. Here, we report a new approach for rapid and accurate G4P quantification which relies on the use of synthesized stable isotope-labelled as internal standards. We anticipate that this approach will accelerate research into the function of G4P signaling in plants, algae and other organisms.

【 授权许可】

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