Nanopore with transverse nanoelectrodes for electrical characterization and sequencing of DNA

SENSORS AND ACTUATORS B-CHEMICAL

卷:132

Article

Gierhart, Brian C.²  Flowitt, David G.³  Chen, Shiahn J.¹  Zhu, Zhineng⁴  Kotecki, David E.⁴  Smith, Rosemary L.^1,4  Collins, Scott D.¹ 

[1] Univ Maine, Surface Sci & Technol Lab, MicroInstruments & Syst Lab, Orono, ME 04469 USA

[2] Univ Calif Davis, Dept Elect & Comp Engn, Davis, CA 95616 USA

[3] Univ Calif Davis, Dept Chem Engn & Mat Sci, Davis, CA 95616 USA

[4] Univ Maine, Dept Elect & Comp Engn, Orono, ME 04469 USA

关键词: nanopore;    nanoelectrodes;    DNA sequencing;    gold nanoparticles;   

DOI  :  10.1016/j.snb.2007.11.054

来源: Elsevier

【 摘 要 】

A DNA sequencing device which integrates transverse conducting electrodes for the measurement of electrode currents during DNA translocation through a nanopore has been nanofabricated and characterized. A focused electron beam (FEB) milling technique, capable of creating features on the order of 1 nm in diameter, was used to create the nanopore. The device was characterized electrically using gold nanoparticles as an artificial analyte with both DC and AC measurement methods. Single nanoparticle/electrode interaction events were recorded. A low-noisie, high-speed transimpedance current amplifier for the detection of nano- to picoampere currents at microsecond time scales was designed, fabricated and tested for future integration with the nanopore device. (C) 2007 Elsevier B.V. All rights reserved.

【 授权许可】

Free